Membrane electroporation and direct gene transfer
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چکیده
منابع مشابه
JEC BB 01516 Membrane electroporation and direct gene transfer l
The direct transfer of genetic material into cells by electroporation can be described in physicochemical terms as an electroporation-resealing hysteresis. The hysteresis concept includes unidirectional state transitions of the membrane, coupled to electrodiffusive migration of DNA through cell wall structures and electroporated plasma membranes. Deeper insight into electroporation phenomena su...
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Gene delivery to adult skeletal muscle is a promising strategy for myofiber signal transduction studies and for the treatment of muscle disorders. Among the nonviral techniques for gene transfer in vivo, electric field applied to the direct injection of plasmid DNA into muscle is safe, inexpensive and simple. However, present DNA delivery technologies should be improved with regard to the level...
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CONTEXT Understanding gene function in the developing pancreas is a major issue for pancreatic cell therapy. The in vivo analysis of gene function has essentially been performed by modulating gene expression in transgenesis. A faster and easier method is electroporation of mouse embryos. This technique, coupled with whole embryo culture, enables one to deliver genes and analyze their effects in...
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The mammalian inner ear forms from a thickened patch of head ectoderm called the otic placode. The placodal ectoderm invaginates to form a cup whose edges cinch together to establish a fluid-filled sac called the otic vesicle or otocyst. The progenitor cells lining the otocyst lumen will give rise to sensory and non-sensory cells of the inner ear. These formative stages of inner ear development...
متن کاملEx ovo electroporation for gene transfer into older chicken embryos.
In ovo electroporation is an excellent method to ectopically induce or inhibit gene expression in chicken embryos and to study the in vivo function of genes during embryonic development. However, the application of electroporation in ovo to date is limited to an early stage of incubation (< stage 20). In older embryos (> stage 22), the vitelline and allantoic vessels have developed extensively ...
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ژورنال
عنوان ژورنال: Bioelectrochemistry and Bioenergetics
سال: 1992
ISSN: 0302-4598
DOI: 10.1016/0302-4598(92)80017-b